DNA Profile Analysis for Primates
- Parentage Verification
- Estimation of Genetic Variation
- Estimation of Relatedness
- Individual Identification
- Population / Subspecies Differentiation
Primate Genetic Analysis
The ability to address questions of genetic identity in
non-human primates is now dramatically enhanced. The DNA profile test offered by Therion
International is more powerful than conventional blood protein analysis because it detects
DNA-sequence information which is highly variable. This technology provides a sensitive
method for sire/dam verification and population genetic analysis.
Following are descriptions of several of the many primate
genetics projects which have been conducted by Therion.
|A wild female baboon was
observed to have consorted with seven males during her receptive period. To verify
paternity, DNA profiles were produced from the female, her offspring and the seven males
with which she had consorted. The DNA probe used was OPT-02 and a portion of the
autoradiograph is shown at left.
Several genetic markers (bands) found in the DNA
profile of the offspring were not present in the DNA profile of the mother. One genetic
marker (yellow bands indicated at left) appeared in the profiles of potential sires C and
D. A second genetic marker (green bands indicated at left) was observed in the DNA profile
of sire D but not in sire C. Other genetic markers and additional DNA probe assays
corroborated the conclusion that sire D was the true sire of this offspring.
Estimation of Genetic Variation and Relatedness Among Individuals
|To ensure the long-term survival and production
of captive colonies of primates it is essential to maximize genetic variation through
rigorously designed breeding programs. Pictured to the right are two sets of DNA profiles
(probe OPT-05) generated from individuals belonging to two separate colonies of New
World primates. Population A exhibits high levels of genetic variation as indicated by a
low level of band sharing among individuals. In contrast, population B exhibits a low
level of genetic variation as indicated by high levels of band sharing among individuals.
Note the nearly identical DNA profile banding patterns. To estimate relatedness among
individuals, similarity indices can be generated from band-sharing coefficients for each
dyadic comparison of individuals within the population.
Estimation of Indices of Population Genetics
|As has been reported recently in the scientific
literature, the results from multi-locus DNA profiles can be used to generate estimates of
classical indices of population genetics including heterozygosity and genetic distance.
The table at left depicts genetic data from a captive population of 28 cynomolgus
macaques. The table at right lists the expected heterozygosity and number of alleles per
locus for ten captive colonies of rhesus macaques. Estimates of genetic distance among
colonies can also be calculated to enhance potential cross colonies breeding management
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